This study is concerned with cytochrome P450 contents and different forms of cytochrome P450 after administration of hydrocortisone in common bile duct ligated and afirenalectomized rats. Content of total hepatic "cytochrome P -450 is, decreased after either common bile duct ligation or adrenalectomy. In contrast, hydrocortisone increases content of hepatic cytochrome P -450.
Sodium dodecyl sulfate -polyacrylamide gel electrophoresis was used to examine the polypetide pattern of rat liver endoplasmic reticulum membrane fraction. This report describes the chromatographic separation of four forms of microsomal cytochrome P -450 apoproteins, whose molecular weights ranging from 50,000 to 58,000, indentified in a sodium dodecyl su-Hate (SDS)_6M urea polyacrlamide gel electrophoresis system. Bile duct ligation was associated with a marked reduction in band Ill and slight reduction in band I while and I,
III and jp decreased with adrenalectomy. Bile duct ligation plus adrenalectomy was associated with a reduction in band I, II, I[ and ITT. And administration of hydrocortisone after bile duct ligation plus adrenalectomy demonstrated a return of band Il and 1U. Hydrocortisone administrated alone increased band U[, while band 1 was decreased.
|